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High Performance Liquid Chromatography (HPLC)

Principles, Instrumentation, Method Development, Validation & Advanced Troubleshooting

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Why Choose Us

The High Performance Liquid Chromatography (HPLC) training course equips laboratory professionals to separate, identify and quantify chemical compounds within diverse, complex mixtures. It develops expertise in an analytical technique that is critical to modern laboratory practice. HPLC is indispensable across pharmaceuticals, biotechnology, environmental monitoring, petrochemicals, food and beverage, and chemical manufacturing. Participants gain the practical understanding required to apply this technique accurately in these varied industrial settings.

Within today’s competitive and regulatory environment, participants study HPLC system operation alongside chromatographic theory, method development and data interpretation. The course combines theoretical foundations with practical work covering chromatographic principles, system components, column chemistry, method optimisation and troubleshooting. This knowledge supports growing requirements for accuracy, precision and regulatory compliance while helping participants manage the complexities of HPLC technology. By preventing poor methodology and system failures that may cause costly errors, failed audits and rework, participants can confidently improve data quality, streamline processes and maintain operational efficiency.

This High Performance Liquid Chromatography (HPLC) training course will highlight:

  • Applying chromatographic theory and principles
  • Operating HPLC systems and understanding their components
  • Using column chemistry to separate compounds in complex mixtures
  • Developing and optimising chromatographic methods
  • Interpreting data for accurate and precise results
  • Troubleshooting poor methodology and system failures
Why Choose High Performance Liquid Chromatography (HPLC)
Course Goals

What are the Goals?

Upon completing the High Performance Liquid Chromatography (HPLC) Training Course, participants will:

  • Acquire a sound knowledge of liquid chromatography principles.
  • Be able to recognise and describe the components of an HPLC system.
  • Become proficient in choosing suitable columns, mobile phases, and detectors.
  • Create, refine, and validate HPLC methods for a range of applications.
  • Use HPLC systems safely and precisely.
  • Interpret chromatographic data and results accurately.
  • Resolve both common and advanced HPLC problems.
  • Maintain compliance with regulatory requirements and data integrity standards.

Who is this Training Course for?

This High Performance Liquid Chromatography (HPLC) training course is designed for:

  • Analytical Chemists
  • HPLC Analysts
  • Quality Control Analysts
  • Pharmaceutical Analysts
  • Method Development Scientists
  • Method Validation Scientists
  • Research and Development Chemists
  • Laboratory Technicians
  • Environmental Laboratory Analysts
  • Food and Beverage Laboratory Analysts
  • Forensic Laboratory Analysts
Learning Approach

How will this Training Course be Presented?

The HPLC Training Course will use a combination of learning approaches to achieve the highest levels of participation and comprehension. These include:

  • Instructor-led lectures covering theoretical concepts.
  • Participative group discussions to strengthen understanding.
  • Practical hands-on sessions for applying theoretical knowledge.
  • Real-life case studies illustrating complex HPLC applications.

This varied approach ensures that participants can put their newly acquired skills into practice immediately and build confidence in operating HPLC systems for routine and advanced analytical tasks.

The Course Content

Fundamentals of Liquid Chromatography & HPLC Systems

  • Introduction to chromatography principles
  • Types of chromatography and applications
  • Basic theory of separation
  • Overview of HPLC techniques (normal phase, reversed phase, ion exchange, size exclusion)
  • HPLC system architecture and workflow
  • Mobile phase delivery systems and pumps
  • Sample introduction and injection systems
  • Safety considerations in HPLC laboratories

Columns, Mobile Phases & Detectors

  • Column chemistry and stationary phases
  • Column selection based on analyte properties
  • Column dimensions and performance parameters
  • Mobile phase composition and solvent selection
  • Buffer preparation, pH control, and solvent compatibility
  • Isocratic vs gradient elution
  • Detectors used in HPLC
  • Detector selection and optimization

Method Development & Optimization

  • Principles of HPLC method development
  • Defining analytical objectives and performance criteria
  • Sample preparation techniques and challenges
  • Optimization of separation parameters
  • Improving resolution and peak symmetry
  • Reducing analysis time and solvent consumption
  • Robustness and ruggedness considerations
  • Method transfer between instruments and laboratories

Method Validation, Data Integrity & Compliance

  • Method validation requirements and guidelines
  • Validation parameters
  • System suitability testing
  • Calibration and standardization
  • Data acquisition and chromatographic integration
  • Data integrity principles and best practices
  • Documentation, reporting, and audit readiness
  • Quality assurance and regulatory expectations

Troubleshooting, Maintenance & Advanced Applications

  • Systematic HPLC troubleshooting approach
  • Common problems and solutions
  • Column care, cleaning, and storage
  • Preventive maintenance of HPLC systems
  • Advanced HPLC applications and case studies
  • Best practices for long-term system performance
  • Course review and practical discussion
Recognition

Certificate

  • • Wallstreet Development Academy Certificate of Completion for delegates who attend and complete the training course.

Frequently Asked Questions

You will learn about HPLC principles, instrumentation, method development, validation and advanced troubleshooting. The course connects the scientific foundations of HPLC with the practical processes used to develop and validate methods. It also addresses how to investigate more complex HPLC problems.
Yes, HPLC instrumentation is a specific part of the course. The training examines instrumentation in the context of chromatographic principles and practical HPLC work. This knowledge supports the later study of method development, validation and troubleshooting.
Yes, the course includes HPLC method validation. Validation is addressed as part of the broader progression from HPLC principles and instrumentation to method development. The course therefore presents validation within the overall analytical method workflow.
The course connects HPLC theory with method work by moving from principles and instrumentation into method development and validation. Advanced troubleshooting is included to show how these areas relate when analytical problems occur. This creates a joined-up view of the HPLC process rather than treating each topic separately.
The course covers the core principles of High Performance Liquid Chromatography. These principles provide the foundation for understanding HPLC instrumentation, developing methods and addressing analytical problems. The course then builds on this foundation through validation and troubleshooting topics.
Yes, HPLC method development is one of the course’s main areas of focus. The course places method development alongside chromatographic principles and instrument knowledge. It also links developed methods to validation and advanced troubleshooting.
The course builds skills for advanced HPLC troubleshooting. It approaches troubleshooting using knowledge of chromatographic principles, instrumentation, method development and validation. This integrated focus helps participants examine HPLC problems in relation to both the system and the analytical method.
This course is relevant to people seeking structured knowledge of HPLC principles, instrumentation, method development, validation and advanced troubleshooting. It may suit those who need to understand how these areas connect in HPLC work. No specific job roles or entry requirements are stated in the provided course information.

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